QTL analysis of seed dormancy in Arabidopsis using recombinant inbred lines and MQM mapping

QTL Mapping: Overview and Using Inbred Lines

While the machinery of quantitative genetics can blissfully function in complete ignorance of any of the underlying genetic details, we are certainly interested (at least one some level) on the genetic basis of trait variation. At the most practical level, if a gene of major effect is segregating in our population of interest, we would certainly like to not only to be aware of this, but also to...

QTL Mapping in New Arabidopsis thaliana Advanced Intercross-Recombinant Inbred Lines

BACKGROUND Even when phenotypic differences are large between natural or domesticated strains, the underlying genetic basis is often complex, and causal genomic regions need to be identified by quantitative trait locus (QTL) mapping. Unfortunately, QTL positions typically have large confidence intervals, which can, for example, lead to one QTL being masked by another, when two closely linked lo...

Mapping Isoflavone QTL with Main, Epistatic and QTL × Environment Effects in Recombinant Inbred Lines of Soybean

Soybean (Glycine max (L.) Merr.) isoflavone is important for human health and plant defense system. To identify novel quantitative trait loci (QTL) and epistatic QTL underlying isoflavone content in soybean, F5:6, F5:7 and F5:8 populations of 130 recombinant inbred (RI) lines, derived from the cross of soybean cultivar 'Zhong Dou 27' (high isoflavone) and 'Jiu Nong 20' (low isoflavone), were an...

Tutorial - Multiple-QTL Mapping (MQM) Analysis

Lecture 9 QTL Mapping 1: Overview and Using Inbred Lines

While the machinery of quantitative genetics can blissfully function in complete ignorance of any of the underlying genetic details, we are certainly interested (at least one some level) on the genetic basis of trait variation. At the most practical level, if a gene of major effect is segregating in our population of interest, we would certainly like to not only to be aware of this, but also to...